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Undergraduate Research Project Management System

Neural expression of MRF4 in Xenopus laevis

Status Current
Seeking Researchers Yes
Start Date 09/01/2007
End Date 06/30/2020
Funding Source UAA Chancellor's Fund; WWAMI program
Funding Amount
Community Partner
Related Course
Last Updated 07/05/2008 01:25AM
Keywords muscle, brain

People

Faculty
  Tim Hinterberger

Student Researchers
  Christy Strong, Jessica Cheatwood

Abstract

Skeletal muscle embryonic development is regulated by many genes, including one called MRF4. In mammals, MRF4 is expressed only in myogenic cells, but in the frog Xenopus laevis, we have observed MRF4 expression in embryonic neural structures as well. This project aims to suppress MRF4 expression in the embryo via antisense morpholino oligonucleotide injection and then assess abnormalities in muscle and neural development anatomically and at the level of gene transcription. Techniques used will include in situ hybridization, RT-PCR, and frozen sectioning of embryos.

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